Simultaneous multicolour imaging for successful experiments.
Multiplexing, or multicolour microscopy, in live cell imaging allows the observation of multiple cell structures and processes in synchrony, providing more physiologically relevant results.
Download this ebook to discover some key aspects of setting up a successful multicolour live cell experiment as well as avoiding some of the major pitfalls. Plus, find out how Leica Microsystems Mica imaging Microhub is highly adapted for live cell imaging experiments.
Key learnings:
Selecting the right fluorophore combinations
Avoiding the misleading effects of fluorescence crosstalk
Maintaining near physiological conditions
Considerations for image acquisition.
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