ebook: A clear and easy guide to Luminex^® multiplex immunoassays

Multiplexing allows multiple biological target analytes to be simultaneously examined and quantified in a single sample. It’s a technique widely used by scientific researchers of all disciplines and provides many benefits.

A Luminex assay is a magnetic microparticle-based immunoassay which utilises the same sandwich principles as traditional ELISAs. Luminex® multiplex immunoassays allow researchers to quantify up to 100 biomarkers with less sample volume than a traditional plate-based ELISA. Color-coded microspheres, or beads, are internally dyed with different proportions of red and infrared fluorophores that correspond to a distinct spectral signature, or bead region. The quantification of multiple cytokines and other biomarkers in a sample provides critical information about biological processes and diseases.