Michael W. Pfaffl (TUM School of Life Sciences)

Valid biomarker signatures from liquid biopsies – how to standardise NGS

6 December 2016 | By Benedikt Kirchner (TUM School of Life Sciences and Ludwig-Maximilians-University Munich), Dominik Buschmann (TUM School of Life Sciences and Ludwig-Maximilians-University Munich), Michael W. Pfaffl (TUM School of Life Sciences)

The advent of next-generation sequencing (NGS) techniques has revolutionised transcriptomics research and opened numerous avenues for scientific and clinical applications. While reverse transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) is still considered the gold standard of gene expression analysis, its high throughput, single-nucleotide resolution and ever-plummeting costs have made NGS…