Team reveals miR-29 as regulator of processes for normal brain maturation

A team has discovered that a molecule called microRNA-29 (miR-29) is a powerful controller of brain maturation in mammals. The researchers, led by scientists at the University of North Carolina (UNC) School of Medicine, US, say that deleting miR-29 in mice caused problems very similar to those seen in autism, epilepsy and other neurodevelopmental conditions.

According to the team, the findings illuminate an important process in the normal maturation of the brain and point to the possibility that disrupting this process could contribute to multiple human brain diseases.

“We think abnormalities in miR-29 activity are likely to be a common theme in neurodevelopmental disorders and even in ordinary behavioural differences in individuals,” said senior author Professor Mohanish Deshmukh. “Our work suggests that boosting levels of miR-29, perhaps even by delivering it directly, could lead to a therapeutic strategy for neurodevelopmental disorders such as autism.”

Each microRNAs (miRNAs) can bind directly to an RNA transcript from certain other genes, preventing it from being translated into a protein. MiRNAs thus effectively serve as inhibitors of gene activity and the typical miRNA regulates multiple genes in this way so that genetic information is not overexpressed. 

The researchers from the study investigated miRNAs involved in the maturation of the brain after birth, a phase that in humans includes approximately the first 20 years of life. When the scientists looked for miRNAs with more activity in the adult mouse brain than the young mouse brain, they found that levels of the miR-29 family were 50 to 70 times higher in the adult mouse brains than in young mouse brains.

The researchers examined a mouse model in which the genes for the miR-29 family were deleted just in the brain. They observed that although the mice were born normally, they soon developed a mix of problems, including repetitive behaviours, hyperactivity and other abnormalities typically seen in mouse models of autism and other neurodevelopmental disorders. Many developed severe epileptic seizures.

The scientists examined gene activity in the brains of the mice, comparing it to activity in mouse brains that had miR-29. As expected, many genes were much more active when miR-29 was no longer there to block their activity. However the team unexpectedly found a large set of genes – associated with brain cells – that were less active in miR-29’s absence.

The researchers found that this was due to one of the target genes that miR-29 normally blocks encoding for an enzyme called DNMT3A. This enzyme places special chemical modifications called CH-methylations onto DNA, to silence genes in the vicinity. In mice brains, the activity of the gene for DNMT3A normally rises at birth and then sharply declines several weeks later. The scientists found that miR-29, which blocks DNMT3A, is what normally forces this sharp decline.

Thus, in the mice whose brains lack miR-29, DNMT3A is not suppressed and the CH-methylation process continues abnormally and many brain cell genes that should become active continue to be suppressed instead. Some of these genes and the gene for DNMT3A itself have been found to be missing or mutated in individuals with neurodevelopmental disorders such as autism, epilepsy and schizophrenia.

To confirm DNMT3A’s role, the scientists created a unique mouse model that prevents miR-29 from suppressing DNMT3A, but leaves miR-29’s other targets untouched. They showed that this unleashing of DNMT3A on its own results in many of the same problems such as seizures and early death, as seen in the mice without miR-29.

The findings highlight and clarify what seems likely to be a crucial process in shaping the brain late in its development: the switching-off of DNMT3A to free up many genes that are meant to be more active in the adult brain.

“These results are the first to identify miR-29 as an essential regulator of CH methylation and to show why restricting CH methylation to a critical period is important for normal brain maturation,” Deshmukh said.

The team are now following up by studying in more detail how the lack of miR-29 in different sets of brain cells might give rise to such disorders and more generally they are studying how miR-29’s activity is regulated in childhood to fine-tune brain functions.

The results are published in Cell Reports.