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Three-dimensional cell cultures (spheroids, organoids) are becoming widely used as a new predictive tool in early drug discovery. The use of 3D cell cultures is believed to provide a more physiologically relevant response than monolayer (2D) cell cultures because they closely mimic the extracellular matrix and cell-cell interactions that occur…
The basic premise of drug discovery screening necessitates that the biological assays upon which it depends can be performed in a reproducible manner. In addition, the techniques employed must generate results that are biologically relevant and actionable.
With breakthroughs in molecular engineering and antibody humanisation, monoclonal antibodies (mAb) are one of the fastest-growing classes of biopharmaceuticals for multiple clinical indications including cancer, cardiovascular disease, autoimmune disorders and infectious disease. Most therapeutic antibody candidates are initially generated using hybridoma technology or primary B-cell screening after antigen immunisation.
Live-cell analysis is now a well-established method that is part of the everyday cell biologist’s armoury for understanding cell biology. For over a decade, the functionality, throughput, and ease of use offered by real-time live-cell analysis has provided a platform for accurate and reproducible cell biology analysis in multiple biomedical…
Stem cells transformed into 3D human liver tissue by scientists show promising support of liver function when implanted into mice with a liver disease...
Drug discovery is a lengthy process that proceeds through several stages. High-throughput screening (HTS) utilising whole-cell-based screening assays plays a fundamental role as a starting point for identifying novel compounds in the drug discovery process.
Drugs of biological origin form the backbone of the drug discovery pipelines for many major pharmaceutical companies. These biologic therapeutics can be defined as proteins derived from eukaryotic cell culture processes. The ability of eukaryotic cells to undertake complex post-translational modifications makes them ideal vehicles for manufacturing proteins emerging from…
Complex biology is a discipline acknowledging that performing biological experiments in vitro should take account of the complexity of the biological context.1 While this may be a noble aim, it has proven difficult to incorporate these elements into the drug discovery process, especially at the high-throughput screening (HTS) stage.2
A new study describes an assay, anchored multiplex PCR (AMP)-based targeted next-generation-sequencing (NGS), with superior diagnostic utility compared to conventional techniques...
A protein identified in the signalling pathway for haemoglobin production has been identified as a possible target to treat sickle cell disease (SCD)...
Researchers have described a new method for culturing Treponema pallidum, that until now it couldn't be cultured in a lab dish...
The Nobel Prize-winning observations and discoveries of John B. Gurdon and Shinya Yamanaka have ignited an explosion of excitement around the potential use of stem cells in research and treatment of human disease.
Human primary hepatocytes (hphep cells) are the gold standard for in vitro evaluation of drug metabolism, drug-drug interactions, safety assessment of drug candidates, and disease modeling.
For the next generation of cell biologists, 3D cell culture and analysis has opened doors to taking applied research one step further.
How to perform successful long term live cell imaging in a high-content analysis system.
