Whitepaper discusses strategies to optimize various factors that cause off-target events with Invitrogen™ TrueCut™ HiFi Cas9 Protein.
CRISPR-Cas9 genome editing is one of the most popular gene therapy tools due to its simplicity, but its successful application depends on many factors. While refinements in the delivery and localisation of nucleases have steadily improved on-target editing efficiency, off-target effects continue to be a problem. To address these issues, we set out to identify a Cas9 variant that would limit the number of off-target edits. See superior results with Invitrogen™ TrueCut™ HiFi Cas9 Protein.
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