Application note: Cellular and biochemical HTRF assays measured in 1536-well microplates
Posted: 30 July 2018 | BMG Labtech GmbH | No comments yet
The PHERAstar FSX microplate reader has been developed to meet the requirements for advanced high throughput screening. The new laser for Time-Resolved-Fluorescence (TRF) assays enables fastest read times for measurement in 1536-well microplates while keeping an excellent assay performance…
Two HTRF assays from Cisbio Bioassays were selected to highlight the enhanced performances of the PHERAstar FSX.
The HTRF IP-One terbium cellular assay can be used to study the effect of GPCR agonist and antagonists on the Gq pathway. The second assay detects the interaction of a protein binding domain (BRD4) with a peptide (GST). Both assays have been miniaturized into the 1536-well microplate format. All liquid handling has been done with the Certus nano liquid dispenser from Gyger.
The PHERAstar FSX offers robust and fast readings for HTS and compound follow-up:
- a full 1536-well plate is measured in 36 seconds (using TRF laser)
- Z´> 0.8 for cellular and biochemical HTRF assay
The rest of this content is restricted - login or subscribe free to access
Thank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.
Why subscribe? Join our growing community of thousands of industry professionals and gain access to:
- quarterly issues in print and/or digital format
- case studies, whitepapers, webinars and industry-leading content
- breaking news and features
- our extensive online archive of thousands of articles and years of past issues
- ...And it's all free!
Click here to Subscribe today Login here
Related content from this organisation
- Product hub: Targeted protein degradation – next-generation therapeutics
- ebook: Binding kinetics in drug discovery
- Application note: Competitive high-throughput analysis of kinase inhibitor binding kinetics
- Product hub: High-throughput screening of cyclic peptide libraries for developing drugs to challenging targets
- Application note: Screening for optimal association and dissociation rates of ligands using a live cell binding assay
Related organisations
BMG Labtech GmbH