Whitepaper: IHC/ICC Protocol Guide 0SHARES Share via Pinterest Reddit Buffer Xing WhatsApp Flipboard Posted: 22 July 2019 | Bio-Techne | No comments yet Immunohistochemistry (IHC) and immunocytochemistry (ICC) are techniques employed to localize antigen expression and are dependent on specific epitope-antibody interactions. IHC refers to the use of tissue sections, whereas ICC describes the use of cultured cells or cell suspensions. In both methods, positive staining is visualized using a molecular label, which can be fluorescent or chromogenic. Briefly, samples are fixed to preserve cellular integrity and then subjected to incubation with blocking reagents to prevent non-specific binding of the antibodies. Samples are subsequently incubated with primary and secondary antibodies, and the signal is visualized for microscopic analysis. To read this whitepaper in full, please complete the form below. By clicking submit you confirm that you accept our terms and conditions and privacy policy. First Name * Surname * Job Title * Organisation * Town/City * Country * Email * Telephone * * This content is provided to you for free thanks to the kind support of our sponsor: Bio-Techne I am happy for my details to be shared with the sponsor(s) of this content *—Please choose an option—YesNo Related content from this organisation Whitepaper: Empower CRISPR cell engineering with single cell sorting Application note: Simplify the qualification of your GMP proteins Whitepaper: Serum-free and Animal-free Research Application note: Single Cell Deposition Efficiency Whitepaper: PSMA Protein in the Battle Against Cancer Related topicsAntibodies, Cell Cultures, Immunology Related organisationsBio-Techne
webinar Advancing ADC Development: End-to-End Solutions from Design to Characterisation By Eurofins Discovery
webinar Solving the AOC puzzle: Strategies for chemistry, manufacturing and regulatory success By Abzena