Application note: Comparison of fully porous and superficially porous particles for the analysis of synthetic oligonucleotides

Superficially porous particle technologies provide a reduced diffusion path in comparison with their fully porous counterparts minimising the resistance to mass transfer during chromatographic separation.

With these particles, one can benefit from their greater efficiency either through improved separation or achieving comparable efficiency with a larger particle size. This enables the method flexibility to increase flow rate. When this is coupled with the benefits of bio-inert hardware, a greater level of separation of oligonucleotide impurities can be observed compared to a sub-2µm fully porous hybrid silica.