Application note: The effect of column hardware on the analysis of synthetic oligonucleotides by LC-MS

Therapeutic oligonucleotides represent a recent breakthrough in the pharmaceutical industry.

Improved reliability to synthetic routes coupled with new drug delivery technologies has given a heightened level of interest in these clinically interesting targets. Chromatographically, they present a challenge due to their polar nature and phosphodiester backbone. As such, ion-pair reversed phase chromatography (IP-RPLC) is necessary, typically using mobile phase consisting of an alkylamine ion pair and perfluorinated alcohol such as HFIP.