Application note: Multiplex Fluorescent Western Blot Detection
Challenge: One touch imaging of multiple proteins on the same blot with NIR fluorescent tags. Solution? Automated imaging with the UVP ChemStudio.
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Challenge: One touch imaging of multiple proteins on the same blot with NIR fluorescent tags. Solution? Automated imaging with the UVP ChemStudio.
Proper signal visualization, detection, and quantification are paramount to the acquisition of accurate and reproducible data...
Cell lysates can be prepared using a variety of methods including physical disruption, reagent-based lysis, or a combination.
Challenge: Non-invasive imaging of a patient-derived orthotropic mouse model of pancreatic cancer expressing GFP. The solution? In vivo imaging.
In vivo imaging has been an indispensable and powerful tool in biomedical research, enabling a number of significant breakthrough discoveries.
The Simoa CorPlex Human Cytokine Panel 1 is a multiplex immunoassay for the quantitative measurement of 10 inflammatory biomarkers in serum and EDTA plasma.
MTT and Cell Cytotoxicity assay kits from Abcam can both be used to measure cell proliferation (viable cells) and cytotoxicity. The choice of which assay to use is mainly based on the desired workflow and time required to complete an assay.
Find out how to accelerate and improve selective single cell cloning with advanced microfluidic dispensing.
Learn how to quantitate a cellular protein sample with BCA protein assay kits on the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices.
Developing more complex, biologically relevant, and predictive cell-based assays for compound screening is a primary challenge in drug discovery.
Macrophages originate from blood monocytes that leave the circulation and penetrate various tissues where they differentiate into macrophages. They are involved in the removal of pathogens and phagocytosis of dead cells. In addition, they initiate inflammation by releasing cytokines that activate vascular cells and facilitate macrophage adhesion to blood vessels…
Understanding the impact of drugs on the cardiac system early in the development process is critical to improving the safety of drugs getting to market.
Automated fluorescence imaging and quantification of cell viability, with EVOS cell imaging system
High-throughput fluorescence imaging and analysis of spheroids - tips, tricks, and tools to efficiently grow, stain, image, and analyze 3D cell cultures
Scientists studying particular disease states often search cellular signaling pathways to gain insight into DNA, RNA, and protein function.